This affinity‐purified polyclonal antiserum was specifically prepared to provide broad cross‐reactivity with human insulin
and common insulin analogs to allow use in ELISAs/RIAs and as an immunoaffinity capture reagent in LC‐MS/MS assays.
The immunogen consisted of pharma‐grade insulin. Following immunization and boosting, the guinea pig antiserum was
purified by a multi‐stepped process which culminated in an affinity column procedure to yield the broad‐specificity
antiserum. This antiserum will work exceptionally well as a capture or detection reagent in a conventional noncompetitive
ELISA format. Because of its polyclonal nature, the antiserum can be used as both a capture and detection reagent. We
recommend either biotinylation or another conjugation procedure be used for the detection reagent. Suggested starting
dilutions are: ELISA: 1/10,000 – 1/50,000, RIA: 1/200,000, and Immunoaffinity capture: 1/10,000 – 1/50,000