Affinity-Purified Guinea Pig Broad Specificity Anti-­Human Insulin (PAb)

$1,000.00

Concentration: 0.5 mg/mL

Volume: 0.2 mL
Amount: 100 µg

Product ID#: RE-01-03-01-01

Description

This affinity‐purified polyclonal antiserum was specifically prepared to provide broad cross‐reactivity with human insulin and common insulin analogs to allow use in ELISAs/RIAs and as an immunoaffinity capture reagent in LC‐MS/MS assays. The immunogen consisted of pharma‐grade insulin. Following immunization and boosting, the guinea pig antiserum was purified by a multi‐stepped process which culminated in an affinity column procedure to yield the broad‐specificity antiserum. This antiserum will work exceptionally well as a capture or detection reagent in a conventional noncompetitive ELISA format. Because of its polyclonal nature, the antiserum can be used as both a capture and detection reagent. We recommend either biotinylation or another conjugation procedure be used for the detection reagent. Suggested starting dilutions are: ELISA: 1/10,000 – 1/50,000, RIA: 1/200,000, and Immunoaffinity capture: 1/10,000 – 1/50,000

Product Data Sheet

Additional information

Concentration

0.5 mg/ml

Volume

0.2 mL

Amount

100 µg

Host

Guinea Pig

Isotype

IgG

Clonality

Polyclonal

Clone

NA

Conjugate

Unconjugated

Form

Liquid

Storage buffer

25mM MES, 0.1 M NaCl, 33% glycerol pH 6.2

Storage Conditions (C)

4, -20

Purification

Affinity